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phh1 grna plasmid addgene cat  (Addgene inc)


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    Structured Review

    Addgene inc phh1 grna plasmid addgene cat
    Phh1 Grna Plasmid Addgene Cat, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 496 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phh1+grna+plasmid+addgene+cat/lentiCRISPRv2+blast+(Plasmid+%2398293)/pm41916278-261-301-303
    Average 96 stars, based on 496 article reviews
    phh1 grna plasmid addgene cat - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Membrane:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Gel Extraction:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    DNA Purification:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Chromatin Immunoprecipitation:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    DNA Sequencing:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Multiplex Assay:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Recombinant:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Plasmid Preparation:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2



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